数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

NE1015 Sigma-AldrichAnti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)

This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-GFAP Cocktail

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, Ca, Ch, Gp, H, M, Porcine, R, Sh	M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
NE1015-100ULCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	100 ul	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
Catalogue Number	NE1015
Brand Family	Calbiochem®
Synonyms	Anti-GFAP Cocktail
Application Data	Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain. Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.

References
References	Vick, W.W., et al. 1987. Acta. Cytol. 31, 816. McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692. Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.

Product Information
Form	Liquid
Formulation	Undiluted ascites.
Positive control	Astrocytes or cytoskeletal preparations
Preservative	≤ 0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	ELISA (1:1000) Frozen Sections (1:1000, see comments) Immunoblotting (1:1000) Immunocytochemistry (1:1000, see comments) Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
Application Comments	This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	purified bovine GFAP protein
Immunogen	Bovine
Clone	SMI-22
Host	Mouse
Isotype	IgG_2b
Species Reactivity	Bovine Canine Chicken Guinea Pig Human Mouse Porcine Rat Sheep
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Upon initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
NE1015-100ULCN	04055977209853

Documentation

Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22) MSDS

职位
物料安全数据表 (MSDS)

Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22) 分析证书

标题	批号
NE1015	输入批号

参考

参考信息概述
Vick, W.W., et al. 1987. Acta. Cytol. 31, 816. McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692. Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-October-2007 RFH
Synonyms	Anti-GFAP Cocktail
Application	ELISA (1:1000) Frozen Sections (1:1000, see comments) Immunoblotting (1:1000) Immunocytochemistry (1:1000, see comments) Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
Application Data	Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain. Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.
Description	Mouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.
Background	Glial fibrillary acidic protein (GFAP) is an intermediate filament protein found only in glial cells or cells of glial origin. It can be detected in astrocytes and certain other astroglia in the CNS, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. GFAP is upregulated and expressed at high levels in astrocytes in many damage and disease states. It is also often highly expressed in neural stem cells and many types of brain tumors.
Host	Mouse
Immunogen species	Bovine
Immunogen	purified bovine GFAP protein
Clone	SMI-22
Isotype	IgG_2b
Species	bovine, canine, chicken, guinea pig, human, mouse, porcine, rat, sheep
Positive control	Astrocytes or cytoskeletal preparations
Form	Liquid
Formulation	Undiluted ascites.
Preservative	≤ 0.1% sodium azide
Comments	This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Upon initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Vick, W.W., et al. 1987. Acta. Cytol. 31, 816. McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692. Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。