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Anti-GHR, mouse monoclonal, clone 3A12, recognizes the GHR protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.
More>>Anti-GHR, mouse monoclonal, clone 3A12, recognizes the GHR protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry. Less<<
Anti-GHR Mouse mAb (3A12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence. Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (1:50)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
Clone
3A12
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-Growth Hormone Receptor
Application
ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (1:50)
Application Data
Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence. Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
Description
Purified mouse monoclonal antibody. Recognizes the GHR protein.
Background
GHR is a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
Clone
3A12
Isotype
IgG2a
Species
human
Positive control
HeLa cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
