Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Anti-GGT1, mouse monoclonal, clone 1F9, recognizes the ~60 kDa GGT1 protein in NIH3T3 cells and human testes tissue. It is validated for Western blotting & immunohistochemistry on paraffin sections.
More>>Anti-GGT1, mouse monoclonal, clone 1F9, recognizes the ~60 kDa GGT1 protein in NIH3T3 cells and human testes tissue. It is validated for Western blotting & immunohistochemistry on paraffin sections. Less<<
Anti-GGT1 Mouse mAb (1F9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of mouse GGT1 by immunoblotting. Sample: Whole cell lysate from NIH/3T3 cells. Primary antibody: Anti-GGT1 Mouse mAb (1F9) (Cat. No. ST1551) (1-5 µg/ml). Detection: chemiluminescence.
Detection of human GGT1 by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human testes tissue. Primary antibody: Anti-GGT1 Mouse mAb (1F9) (Cat. No. ST1551) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa GGT1 protein.
Background
GGT1 catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 381-471 of human GGT1, expressed as a GST fusion protein
Clone
1F9
Isotype
IgG2α
Species
human, mouse
Positive control
NIH3T3 cells, Human testes tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw aliquot and freeze (-20°C).