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ABC519 Sigma-AldrichAnti-FANCF Antibody

Anti-FANCF is an antibody against FANCF for use in WB, ICC.

Anti-FANCF Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FANCF UniProt Number: Q9NPI8

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	ABC519
Description	Anti-FANCF Antibody
Alternate Names	Fanconi anemia group F protein Protein FACF FANCF
Background Information	Fanconi anemia group F protein (FANCF), also known as Protein FACF, and encoded by the gene symbol FANCF, is a DNA repair protein that may operate in a postreplication DNA repair or may be involved in a cell cycle checkpoint function. Recent studies have implicated that FACF may play an important role in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Mutations affecting the FANCF gene have been associated with Fanconi anemia complementation group F (FANCF), a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. This disorder is also associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

References

Product Information
Format	Serum
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-FANCF is an antibody against FANCF for use in WB, ICC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blotting Analysis: A representative lot detected FANCF in 293T cells were cotransfected with HA-tagged HES1 and FA coding vectors, HeLa cell extracts treated with L685,458 , 293T cells were cotransfected with FA gene expression plasmids as indicated with either empty, or BACE1 or HES1 coding vectors, 293T cells were cotransfected with FANCF expression plasmids along with increasing amounts of HES1 coding vector (Tremblay, C.S., et al. (2008). Blood Journal. 112:P2062-2070). Immunocytochemistry Analysis: A representative lot detected FANCF in HeLa cells treated with the gamma-secretase inhibitor L685,458 (Tremblay, C.S., et al. (2008). Blood Journal. 112:P2062-2070).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human FANCF.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_073562
Gene Symbol	FANCF
Purification Method	Unpurified
UniProt Number	Q9NPI8
Molecular Weight	~45 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected FANCF in 10 µg of MCF-7 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABC519	04055977113587

Documentation

Anti-FANCF Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-FANCF Antibody 分析证书

标题	批号
Anti-FANCF	Q2479833
Anti-FANCF - 3275034	3275034
Anti-FANCF - 3478266	3478266
Anti-FANCF -2756656	2756656
Anti-FANCF Polyclonal Antibody	2955305

参考

参考概述	公共医疗ID
HES1 is a novel interactor of the Fanconi anemia core complex. Tremblay, CS; Huang, FF; Habi, O; Huard, CC; Godin, C; Lévesque, G; Carreau, M Blood 112 2062-70 2008 显示摘要 Fanconi anemia (FA) proteins are thought to play a role in chromosome stability and repair of DNA cross-links; however, these functions may not fully explain the developmental abnormalities and bone marrow failure that are characteristic of FA individuals. Here we associate the FA proteins with the Notch1 developmental pathway through a direct protein-protein interaction between the FA core complex and the hairy enhancer of split 1 (HES1). HES1 interaction with FA core complex members is dependent on a functional FA pathway. Cells depleted of HES1 exhibit an FA-like phenotype that includes cellular hypersensitivity to mitomycin C (MMC) and lack of FANCD2 monoubiquitination and foci formation. HES1 is also required for proper nuclear localization or stability of some members of the core complex. Our results suggest that HES1 is a novel interacting protein of the FA core complex.	18550849

参考概述

公共医疗ID

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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