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ABE1379 Sigma-AldrichAnti-F-box/WD repeat-containing protein 7 Antibody

This Anti-F-box/WD repeat-containing protein 7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of F-box/WD repeat-containing protein 7.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: FBXW7, FBW7, FBX30, SEL10 UniProt Number: Q969H0

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABE1379
Description	Anti-F-box/WD repeat-containing protein 7 Antibody
Alternate Names	Archipelago homolog F-box/WD repeat-containing protein 7 hAgo F-box and WD-40 domain-containing protein 7 F-box protein FBX30 SEL-10 hCdc4
Background Information	F-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NFκB2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-F-box/WD repeat-containing protein 7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of F-box/WD repeat-containing protein 7.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected F-box/WD repeat-containing protein 7 in human cerebellum and human pancreas tissue.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human F-box/WD repeat-containing protein 7.
Epitope	Internal
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	Although immunogen sequence is present in all three spliced isoforms, both target band size by Western blotting and cytoplasmic staining pattern by immunohistochemistry are consistent with the detection of isoform 2 (Archipelago beta, FBW7beta, 69K) by this polyclonal antibody.
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to react with Bovine, Canine, Feline, Porcine, Rhesus Macaque, Gorilla, Horse based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_060785
Gene Symbol	FBXW7 FBW7 FBX30 SEL10
Purification Method	Affinity Purfied
UniProt Number	Q969H0
Molecular Weight	~70 kDa observed. 79.7 kDa (isoform 1; Archipelago alpha, FBW7alpha, 110K, common), 70.3 kDa (isoform 2; Archipelago beta, FBW7beta, 69K), and 66.1 kDa (isoform 3; Archipelago gamma, FBW7gamma, Hippocampal) calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MCF-10A human breast epithelial cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected F-box/WD repeat-containing protein 7 in 10 µg of human breast cancer MDA-MB-231 and non-tumorigenic MCF-10A breast epithelial cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABE1379	04055977274813

Documentation

Anti-F-box/WD repeat-containing protein 7 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-F-box/WD repeat-containing protein 7 Antibody 分析证书

标题	批号
Anti-F-box/WD repeat-containing protein 7 -Q2592810	Q2592810

技术信息

标题
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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