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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MAB1072 Sigma-AldrichAnti-Erythropoietin Antibody, clone 1C8

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

Anti-Erythropoietin Antibody, clone 1C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Metabolism Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: EPO, MGC138142, EP UniProt Number: P01588

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ELISA, IF, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB1072
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Erythropoietin Antibody, clone 1C8
Alternate Names	erythropoietin Epoetin epoetin
Background Information	Erythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619).

References

Product Information
Format	Purified
HS Code	3002 15 90
Control	Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Presentation	Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 300 mM NaCl, and 0.02% sodium merthiolate.
Quality Level	MQ100

Applications
Application	Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
Key Applications	ELISA Immunofluorescence Western Blotting
Application Notes	ELISA: For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate. Immunosorbent preparation Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Recombinant human Epo.
Clone	1C8
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Reacts with human Erythropoietin (Epo).
Isotype	IgG2b
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000799.2
Entrez Gene Summary	This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Gene Symbol	EPO MGC138142 EP
Purification Method	Protein A Purfied
UniProt Number	P01588
UniProt Summary	FUNCTION: SwissProt: P01588 # Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. SIZE: 193 amino acids; 21307 Da SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. SIMILARITY: SwissProt: P01588 ## Belongs to the EPO/TPO family.
Molecular Weight	34 kDa (glycosylated)

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on Mouse kidney lysates. Western Blot Analysis: 1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt.

Packaging Information
Material Size	250 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MAB1072	04053252266386

Documentation

Anti-Erythropoietin Antibody, clone 1C8 MSDS

职位
物料安全数据表 (MSDS)

Anti-Erythropoietin Antibody, clone 1C8 分析证书

标题	批号
Anti-Erythropoietin, clone 1C8 - 2121751	2121751
Anti-Erythropoietin, clone 1C8 - 2148240	2148240
Anti-Erythropoietin, clone 1C8 - 2111385	2111385
Anti-Erythropoietin, clone 1C8 - 2197223	2197223
Anti-Erythropoietin, clone 1C8 - 2327716	2327716
Anti-Erythropoietin, clone 1C8 - 2330589	2330589
Anti-Erythropoietin, clone 1C8 - 3184765	3184765
Anti-Erythropoietin, clone 1C8 - 3207103	3207103
Anti-Erythropoietin, clone 1C8 - 4082572	4082572
Anti-Erythropoietin, clone 1C8 - LV1514199	LV1514199

Alternative Packsize

产品目录编号

描述

MAB1072-K

Anti-Erythropoietin, clone H6H7 (1mg)

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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