Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
Clone
3B10
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
04-November-2010 RFH
Synonyms
Anti-ETS Variant 6
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Data
Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence. Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~55-60 kDa ETV6 protein.
Background
ETV6 contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that this protein is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma.
Host
Mouse
Immunogen
Full-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
Clone
3B10
Isotype
IgG₁
Species
human
Positive control
Jurkat cells, Human placenta tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
