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Anti-EPHA4, mouse monoclonal, clone 6H7, recognizes the EPHA4 protein in NIH3T3 cells. It is validated for use in ELISA and Western blotting.
More>>Anti-EPHA4, mouse monoclonal, clone 6H7, recognizes the EPHA4 protein in NIH3T3 cells. It is validated for use in ELISA and Western blotting. Less<<
Anti-EPHA4 Mouse mAb (6H7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
Clone
6H7
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-October-2010 RFH
Synonyms
Anti-Ephrin Receptor A4
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~100 kDa EPHA4 protein.
Background
This protein belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
Clone
6H7
Isotype
IgG₁
Species
human, mouse
Positive control
NIH3T3 cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
