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GR15L
Sigma-AldrichAnti-EGFR (Ab-5) Mouse mAb (EGFR.1)
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.
More>>Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies. Less<<
Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
partially purified, human EGFR from A431 cells
Immunogen
Human
Clone
EGFR.1
Host
Mouse
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Hunter T., 1984. Nature311, 414. Reynolds, F.H., Jr., et al. 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (Cat. No. GR15L) (1 µg/ml). Detection: fluorescence.
Description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Background
Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor alpha to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
Host
Mouse
Immunogen species
Human
Immunogen
partially purified, human EGFR from A431 cells
Clone
EGFR.1
Isotype
IgG2b
Species
human, not mouse, not rat
Positive control
A-431 cells
Negative control
HL-60 cells
Form
Lyophilized
Formulation
Lyophilized from a volatile buffer, 100 µg BSA.
Preservative
None
Comments
Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Toxicity
Standard Handling
References
Hunter T., 1984. Nature311, 414. Reynolds, F.H., Jr., et al. 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Application references
Neutralization Studies
Gill, G., et al. 1984. J. Biol. Chem.259, 7755.
Kawamoto, T., et al. 1984. J. Biol. Chem.259, 7761.
Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1337.
Sato, J.D., et al. 1983. Molec. Biol. Med.1, 511.
