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			96-Well Plate

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GR15 Sigma-AldrichAnti-EGFR (Ab-5) Mouse mAb (EGFR.1)

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Epidermal Growth Factor Receptor

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.
Catalogue Number	GR15
Brand Family	Calbiochem®
Synonyms	Anti-Epidermal Growth Factor Receptor
Application Data	Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (EGFR.1) (Cat. No. GR15L) (1 µg/ml). Detection: flourescence.

References
References	Hunter T., 1984. Nature 311, 414. Reynolds, F.H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.
Negative control	HL-60 cells
Positive control	A-431 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.
Key Applications	Flow Cytometry Immunofluorescence Immunoprecipitation Neutralization Studies
Application Notes	Flow Cytometry (1-5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (1-2 µg/sample) Neutralization Studies (1-2 µg/ml; use Cat. No. GR15L; see application references)
Application Comments	EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	partially purified, human EGFR from A431 cells
Immunogen	Human
Clone	EGFR.1
Host	Mouse
Isotype	IgG_2b
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
GR15-100UGCN	04055977228267

Documentation

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) MSDS

职位
物料安全数据表 (MSDS)

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) 分析证书

标题	批号
GR15	输入批号

参考

参考信息概述
Hunter T., 1984. Nature 311, 414. Reynolds, F.H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	27-August-2007 RFH
Synonyms	Anti-Epidermal Growth Factor Receptor
Application	Flow Cytometry (1-5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (1-2 µg/sample) Neutralization Studies (1-2 µg/ml; use Cat. No. GR15L; see application references)
Application Data	Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (EGFR.1) (Cat. No. GR15L) (1 µg/ml). Detection: flourescence.
Description	Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Background	Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor alpha to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
Host	Mouse
Immunogen species	Human
Immunogen	partially purified, human EGFR from A431 cells
Clone	EGFR.1
Isotype	IgG_2b
Species	human, not mouse, not rat
Positive control	A-431 cells
Negative control	HL-60 cells
Form	Liquid
Formulation	In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Hunter T., 1984. Nature 311, 414. Reynolds, F.H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Application references	Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.

Related Products

产品目录编号

描述

GR15L

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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