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GR13L
Sigma-AldrichAnti-EGFR (Ab-3) Mouse mAb (225)
Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
More>>Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization. Less<<
Anti-EGFR (Ab-3) Mouse mAb (225) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Original Clone
Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1337.
Neutralization Studies
Gill, G., et al. 1984. J. Biol. Chem.259, 7755.
Kawamoto, T., et al. 1984. J. Biol. Chem.259, 7761.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1337.
Sato, J.D., et al. 1983. Biol. Med.1, 511.
Key Applications
Immunoprecipitation
Neutralization Studies
Application Notes
Immunoprecipitation (1-2 µg per sample) Neutralization Studies (2 µg/ml, see comments and application references)
Application Comments
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
partially purified, human EGFR from A431 cells
Immunogen
Human
Clone
225
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Hunter T. 1984. Nature311, 414. Reynolds, F.H., et. al 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
25-October-2007 JSW
Synonyms
Anti-EGF-R
Application
Immunoprecipitation (1-2 µg per sample) Neutralization Studies (2 µg/ml, see comments and application references)
Description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Background
Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF receptor is phosphorylated on tyrosine residues. Subsequent to binding, EGF receptor complexes are internalized and cell proliferation takes place with partially-purified EGF receptor from human A431 epidermal carcinoma cells.
Host
Mouse
Immunogen species
Human
Immunogen
partially purified, human EGFR from A431 cells
Clone
225
Isotype
IgG₁
Species
human, not mouse, not rat
Positive control
A431 cells
Negative control
HL-60 cells
Form
Lyophilized
Formulation
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
Preservative
None
Comments
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Hunter T. 1984. Nature311, 414. Reynolds, F.H., et. al 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Application references
Original Clone
Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1337.
Neutralization Studies
Gill, G., et al. 1984. J. Biol. Chem.259, 7755.
Kawamoto, T., et al. 1984. J. Biol. Chem.259, 7761.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1337.
Sato, J.D., et al. 1983. Biol. Med.1, 511.
