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MABE945 Sigma-AldrichAnti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8

This Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: UHRF1, ICBP90, NP95, RNF106 UniProt Number: Q96T88

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ICC, ChIP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE945
Description	Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8
Alternate Names	E3 ubiquitin-protein ligase UHRF1 Inverted CCAAT box-binding protein of 90 kDa Nuclear protein 95 Nuclear zinc finger protein Np95 HuNp95 hNp95 RING finger protein 106 Transcription factor ICBP90 Ubiquitin-like PHD and RING finger domain-containing protein 1 hUHRF1 Ubiquitin-like-containing PHD and RING finger domains protein 1
Background Information	Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1.
Key Applications	Immunocytochemistry Chromatin Immunoprecipitation (ChIP) Western Blotting
Application Notes	Western Blotting Analysis: A representative lot detected E3 ubiquitin-protein ligase UHRF1 in overexpressed lysate (Martin Walsh, Mount Sinai School of Medicine). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in NTera2 cells (Martin Walsh, Mount Sinai School of Medicine).

Biological Information
Immunogen	Recombinant protein corresponding to human E3 ubiquitin-protein ligase UHRF1.
Clone	7C8
Concentration	1 mg/mL
Host	Mouse
Isotype	IgG2bκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001041666
Gene Symbol	UHRF1 ICBP90 NP95 RNF106
Purification Method	Protein G Purified
UniProt Number	Q96T88
Molecular Weight	90 kDa calculated

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected E3 ubiquitin-protein ligase UHRF1 in HeLa cells. Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE945	04053252992865

Documentation

Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 MSDS

职位
物料安全数据表 (MSDS)

Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 分析证书

标题	批号
Anti-E3 ubiquitin-protein ligase UHRF1, clone 7C8 - Q2454905	Q2454905
Anti-E3 ubiquitin-protein ligase UHRF1, clone 7C8 - 3247501	3247501

技术信息

标题
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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