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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABT155 Sigma-AldrichAnti-Dynamin related protein 1 (Drp1) Antibody

This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Cell Structure Research Sub-Category: Organelle & Cell Markers, Apoptosis - Additional Gene Symbol: Dnm1l, Drp1 UniProt Number: Q8K1M6

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概述

Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Ox, Rhesus Macaque	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABT155
Description	Anti-Dynamin related protein 1 (Drp1) Antibody
Alternate Names	Dynamin-1-like protein Dynamin family member proline-rich carboxyl-terminal domain less Dymple Dynamin-related protein 1
Background Information	Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.

References

Product Information
Format	Affinity Purified
Control	Mouse embryonic stem cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Dynamin related protein 1 in human embryonic stem cell H9 cells.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the GSK3B interaction domain of mouse Dynamin related protein 1 (Drp1).
Epitope	GSK3B interaction domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Dynamin related protein 1 (Drp1) at the GSK3B interaction domain.
Species Reactivity	Human Mouse Rat Ox Rhesus Macaque
Species Reactivity Note	Demonstrated to react with Human and Mouse. Predicted to react with Rat, Ox, and Rhesus Macaque based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_690029
Gene Symbol	Dnm1l Drp1
Purification Method	Affinity Purfied
UniProt Number	Q8K1M6
UniProt Summary	FUNCTION: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport (By similarity). CATALYTIC ACTIVITY: GTP + H2O = GDP + phosphate. SUBUNIT STRUCTURE: Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule By similarity. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria (By similarity). SUBCELLULAR LOCATION: Cytoplasm › cytosol (By similarity). Golgi apparatus (By similarity). Endomembrane system; Peripheral membrane protein. Note: Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex (By similarity). TISSUE SPECIFICITY: Expressed in the cerebellum and in several regions of the cerebrum and diencephalon. Strongly expressed in the cerebellar Purkinje cells and in the pontile giant neurons. DOMAIN: The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization (By similarity). PTM: Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-643 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-622 also promotes mitochondrial fission (By similarity). Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity (By similarity). S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage (By similarity). DISRUPTION PHENOTYPE: In embryonic fibroblasts, mice show defects in trophoblast giant cells and cardiomyocytes. Extensive mitochondrial networks are formed, and peroxisomes elongated. In brain, there are developmental defects in which Purkinje cells contained few giant mitochondria. In the forebrain, there is a decreased number of neurites and defective synapse formation. Embryos undergo developmentally-regulated apoptosis during neural tube formation. Mice die by around embryonic day 12. SEQUENCE SIMILARITIES: Belongs to the dynamin family. Contains 1 GED domain.
Molecular Weight	~80kDa (isoform 2) & ~82 kDa (isoform 1) observed 5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse embryonic stem cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABT155	04053252427145

Documentation

Anti-Dynamin related protein 1 (Drp1) Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Dynamin related protein 1 (Drp1) Antibody 分析证书

标题	批号
Anti-Dynamin related protein 1 (Drp1) - 2352425	2352425
Anti-Dynamin related protein 1 (Drp1) - 2384013	2384013
Anti-Dynamin related protein 1 (Drp1) - 2467136	2467136
Anti-Dynamin related protein 1 (Drp1) - 2109563	2109563
Anti-Dynamin related protein 1 (Drp1) - 2216896	2216896
Anti-Dynamin related protein 1 (Drp1) - 2309307	2309307
Anti-Dynamin related protein 1 (Drp1) - 3211144	3211144
Anti-Dynamin related protein 1 (Drp1) - 3255436	3255436
Anti-Dynamin related protein 1 (Drp1) - 3482837	3482837
Anti-Dynamin related protein 1 (Drp1) - 3674865	3674865

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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