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Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.
More>>Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC. Less<<
Anti-Dopamine D₄ Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Immunoblotting (1:500) Immunohistochemistry (1:10,000)
Application Comments
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-July-2007 RFH
Application
ELISA (see comments) Immunoblotting (1:500) Immunohistochemistry (1:10,000)
Description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
Host
Rabbit
Immunogen species
Human
Immunogen
a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
Isotype
IgG
Species
human, rat
Positive control
Rat brain
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
None
Comments
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
Toxicity
Standard Handling
References
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
