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Anti-Dopamine D2S Receptor, rabbit polyclonal, recognizes the ~48 kDa dopamine D2S receptor. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, and IHC.
More>>Anti-Dopamine D2S Receptor, rabbit polyclonal, recognizes the ~48 kDa dopamine D2S receptor. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, and IHC. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Immunoblotting (1:800) Immunohistochemistry (1:5000)
Application Comments
Does not cross-react with other dopamine receptors. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted serum detected a single band at ~48 kDa in an analysis using whole rat brain homogenates. For immunohistochemistry, a 1:500 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including the plasma membrane of neurons in the cortex and caudate putamen. Staining was also observed along apical dendrites of layer 5 cortex cells. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic (PLKEAARRC) peptide corresponding to amino acids 239-246 of human D2s dopamine receptor
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody, use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
14-October-2010 RFH
Application
ELISA (see comments) Immunoblotting (1:800) Immunohistochemistry (1:5000)
Description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48 kDa dopamine D2s receptor.
Host
Rabbit
Immunogen species
Human
Immunogen
a synthetic (PLKEAARRC) peptide corresponding to amino acids 239-246 of human D2s dopamine receptor
Isotype
IgG
Species
human, rat
Form
Lyophilized
Formulation
Undiluted serum.
Comments
Does not cross-react with other dopamine receptors. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted serum detected a single band at ~48 kDa in an analysis using whole rat brain homogenates. For immunohistochemistry, a 1:500 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including the plasma membrane of neurons in the cortex and caudate putamen. Staining was also observed along apical dendrites of layer 5 cortex cells. Variables associated with assay conditions will dictate the proper working dilution.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody, use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
