数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

324390 Sigma-AldrichAnti-Dopamine D₁ Receptor Rabbit pAb

This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.

Anti-Dopamine D₁ Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the dopamine D₁ receptor in rat brain. Does not cross-react with other dopamine receptors.
Catalogue Number	324390
Brand Family	Calbiochem®

References
References	Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.

Product Information
Form	Lyophilized
Formulation	Undiluted serum.
Positive control	Rat brain
Preservative	None
Quality Level	MQ100

Applications
Application References	Immunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.
Key Applications	Enzyme-Linked Immunosorbent Assay Free-floating Sections Frozen Sections Immunoblotting (Western Blotting) Not Paraffin Sections
Application Notes	ELISA (see comments) Immunoblotting (1:1000) Frozen Sections (1:5000) Free-Floating Sections (1:5000) Paraffin Sections (not recommended)
Application Comments	This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
324390-100ULCN	04055977216516

Documentation

Anti-Dopamine D₁ Receptor Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

Anti-Dopamine D₁ Receptor Rabbit pAb 分析证书

标题	批号
324390	输入批号

参考

参考信息概述
Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.

引用

标题

Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	27-July-2007 RFH
Application	ELISA (see comments) Immunoblotting (1:1000) Frozen Sections (1:5000) Free-Floating Sections (1:5000) Paraffin Sections (not recommended)
Description	Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D₁ receptor protein.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
Isotype	IgG
Species	human, mouse, rat
Positive control	Rat brain
Form	Lyophilized
Formulation	Undiluted serum.
Preservative	None
Comments	This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Toxicity	Standard Handling
References	Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Citation	Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
Application references	Immunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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