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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABC480 Sigma-AldrichAnti-Dido3 Antibody

Anti-Dido3 Antibody is an antibody against Dido3 for use in Western Blotting and Immunocytochemistry.

Anti-Dido3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
技术信息

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DIDO1, C20orf158, DATF1, KIAA0333 UniProt Number: Q9BTC0

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Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABC480
Description	Anti-Dido3 Antibody
Alternate Names	Dido3 Death-inducer obliterator 1 DIO-1 hDido1 Death-associated transcription factor 1 DATF-1
Background Information	Death inducer obliterator 3 (Dido3) is a transcription factor encoded by the DIDO1 (also known as BYE1, DIO1, DATF1, DIDO2, DIDO3) gene in human. Three other isoforms, including Dido1 & 2 (isoform 2 & 1, respectively), are also encoded by the same gene through alternative splicing. In addition to early research activities regarding apoptosis-inducing activity of Dido proteins, later studies reveal important roles of Dido1 & Dido3 (isoform 4) in maintaining stem cell genomic stability and self-renewal (PMID 23831028 & 24347171). Dido3 binds histone H3K4me3 in the nucleus. Histone H3T3 or H3T6 phosphorylation causes H3K4 demethylation and Dido3 translocation from chromatin to the mitotic spindle. Anti-Dido3, Cat. No. ABC480, does not cross-react with Dido1 or Dido2.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ300

Applications
Application	Anti-Dido3 Antibody is an antibody against Dido3 for use in Western Blotting and Immunocytochemistry.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: 0.5 µg/mL from a representative lot detected Dido3 in HT-29 cells.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Dido3 near the C-Terminus.
Epitope	Near C-Terminus
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_149072
Gene Symbol	DIDO1 C20orf158 DATF1 KIAA0333
Purification Method	Affinity Purfied
UniProt Number	Q9BTC0
Molecular Weight	~260 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HT-29 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Dido3 in 10 µg of HT-29 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABC480	04055977158939

Documentation

Anti-Dido3 Antibody 分析证书

标题	批号
Anti-Dido3 -Q2564154	Q2564154

技术信息

标题
White Paper: Further considerations of antibody validation and usage.

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描述

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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