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MABE1905-25UG Anti-DNA ligase I Antibody, clone 5H5

Anti-DNA ligase I, clone 5H5, Cat. No. MABE1905, is a mouse monoclonal antibody that detects DNA ligase 1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABE1905-25UG
25 μg  
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      概述

      Replacement Information
      Description
      Catalogue NumberMABE1905-25UG
      DescriptionAnti-DNA ligase I Antibody, clone 5H5
      Alternate Names
      • DNA ligase 1
      • EC:6.5.1.1
      • Polydeoxyribonucleotide synthase [ATP] 1
      Background InformationDNA ligase 1 (UniProt: P18858; also known as EC:6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1) is encoded by the LIG1 gene (Gene ID: 3978) in human. DNA ligase is the enzyme that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. DNA ligase I joins adjacent Okazaki fragments generated during the lagging strand synthesis and is recruited to replication factories during the S-phase of cell cycle. The recruitment is directed replication factory-targeting sequence (RFTS), which is also shown to mediate its interaction with the proliferating cell nuclear antigen (PCNA). Its C-terminal domain contains the catalytic region and consists of a DNA binding domain (aa 262-534), an adenylation domain (aa 535-747), and an OB-fold domain. The adenylation domain contains five conserved motifs (I, III, IIIa, IV, and V) that are reported to contribute to the surface of nucleotide binding pocket. The N-terminal region is non-catalytic and participates in nuclear localization and DNA replication. Human DNA ligase I can be phosphorylated on serine 66 by casein kinase 2 in a cell cycle-dependent manner. Several other phosphorylation sites have also been reported and the enzyme exits in a hyperphosphorylated state during the M-phase. (Ref.: Vitolo, B., et al. (2005). Eur. J. Histochem. 49(4); 349-354; Ferrari, G., et al. (2003). J. Biol. Chem. 278(39); 37761-3777; Howes, TR., and Tomkinson, AE. (2012). Subcell. Biochem. 62:327-41).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-DNA ligase I, clone 5H5, Cat. No. MABE1905, is a mouse monoclonal antibody that detects DNA ligase 1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected DNA ligase I in Immunohistochemistry applications (Vitolo, B., et. al. (2005). Eur J Histochem. 49(4): 349-54).

      Western Blotting Analysis: A representative lot detected DNA ligase I in Western Blotting applications (Vitolo, B., et. al. (2005). Eur J Histochem. 49(4): 349-54).

      Immunocytochemistry Analysis: A representative lot detected DNA ligase I in Immunocytochemistry applications (Vitolo, B., et. al. (2005). Eur J Histochem. 49(4): 349-54).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged purified human recombinant DNA ligase I overexpressed in a baculovirus/insect cell system.
      Clone5H5
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5H5 is a mouse monoclonal antibody that detects human DNA ligase I.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LIGI
      • LIG1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~100 kDa observed; 101.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected DNA ligase I in HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABE1905-25UG 04061841990192

      Documentation

      Anti-DNA ligase I Antibody, clone 5H5 MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-DNA ligase I Antibody, clone 5H5 分析证书

      标题批号
      Anti-DNA ligase I, clone 5H5 - Q3442996 Q3442996

      参考

      参考概述公共医疗ID
      A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples
      B Vitolo 1 , M R Lidonnici, C Montecucco, A Montecucco
      Eur J Histochem  49(4):  349-54  2005

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