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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABE1950 Sigma-AldrichAnti-DBC1 Antibody

Detect DBC1 using this Anti-DBC1 Antibody validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP).

Anti-DBC1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: CCAR2, DBC1, KIAA1967 UniProt Number: Q8N163

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP, ICC, IHC, ChIP	Rb	Purified	Polyclonal Antibody

Description
Catalogue Number	ABE1950
Description	Anti-DBC1 Antibody
Alternate Names	Cell cycle and apoptosis regulator protein 2 Cell division cycle and apoptosis regulator protein 2 DBIRD complex subunit KIAA1967 Deleted in breast cancer gene 1 protein DBC-1 DBC.1 p30 DBC DBC1
Background Information	Cell cycle and apoptosis regulator protein 2 (UniProt Q8N163; also known as Cell division cycle and apoptosis regulator protein 2, DBC-1, DBC.1, DBIRD complex subunit KIAA1967, Deleted in breast cancer 1, p30 DBC, p30 DBC protein) is encoded by the CCAR2 (also known as DBC1, KIAA1967, NET35) gene (Gene ID 57805) in human. Deleted in breast cancer gene 1 protein (DBC1) is a nuclear protein involved in regulating functions of nuclear receptors (NRs), including androgen receptor (AR) and estrogen receptor (ER) alpha and beta. DBC1 stabilizes the interaction between chicken ovalbumin upstream promoter transcription factor I (COUP-TFI) and NCoR by interacting directly with both proteins. DBC1 also interacts with NR complex interacting factor (NRC)-1 to drive the transcription regulation of NRs. Caspase-dependent processing of DBC1 promotes apoptosis, and depletion of DBC1 negatively regulates p53-dependent apoptosis through its specific inhibition of SIRT1. The N-terimus of DBC1 is shown to interact with BRCA1 C-terminal BRCT domain and the expression of DBC1 represses BRCA1-mediated transactivation of the SIRT1 promoter. DBC1 is also a negative regulator against the ligand-dependent transcriptional activation function of ERbeta. The DBC1 protein encoded by the CCAR2 gene is not the same as the protein encoded by the BRINP1 gene, which also goes by the same acronym DBC1 for "Deleted in bladder cancer protein 1".

References

Product Information
Format	Purified
Presentation	Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect DBC1 using this Anti-DBC1 Antibody validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP).
Key Applications	Western Blotting Immunoprecipitation Immunocytochemistry Immunohistochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Western Blotting Analysis: A representative lot detected DBC1 in HeLa cell lysate (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Western Blotting Analysis: A representative lot detected DBC1 in MDA-MB-231 cell lysate (Koyama, S., et al. (2010). BBRC. 392:357-362). Immunoprecipitation Analysis: A representative lot immunoprecipitated DBC1 in HeLa cell lysate (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Immunocytochemistry Analysis: A representative lot detected DBC1 in MCF7 cells (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Immunocytochemistry Analysis: A representative lot detected DBC1 in MCF7 and T47D cells (Koyama, S., et al. (2010). BBRC. 392:357-362). Immunohistochemistry Analysis: A representative lot detected DBC1 in ductal epithelium and adipose regions of human breast tissue sections (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Immunohistochemistry Analysis: A representative lot detected DBC1 in human breast cancer tissue (Hiraike, H., et al. (2011). Experimental and Therapeutic Medicane. 2:1105-1109). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected DBC1 occupancy at the SIRT1 gene promoter region (1354–1902) known to be targeted by BRCA1 (Hiraike, H., et al. (2010). Br. J. Cancer. 102(6):1061-1067).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human DBC1.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	Expected to reacts with both spliced isoforms.
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_066997
Gene Symbol	CCAR2 DBC1 KIAA1967
Purification Method	Protein A Purfied
UniProt Number	Q8N163
Molecular Weight	~130 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MCF7 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected DBC1 in 10 µg of MCF7 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABE1950	04055977173062

Documentation

Anti-DBC1 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-DBC1 Antibody 分析证书

标题	批号
Anti-DBC1 -Q2573218	Q2573218

技术信息

标题
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics
White Paper: Further considerations of antibody validation and usage.

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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