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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CC12 Sigma-AldrichAnti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet
Citations

同义词: Anti-Bcl-1, Anti-PRAD-1

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
Catalogue Number	CC12
Brand Family	Calbiochem®
Synonyms	Anti-Bcl-1, Anti-PRAD-1

References
References	Inaba, T., et al. 1992. Genomics 13, 565. Jiang, W., et al. 1992. Cancer Res. 2, 2980. Pines, J. 1992. Cell Growth Differ. 2, 305. Xiong, Y., et al. 1992. Genomics 13, 575. Xiong, Y., et al. 1992. Cell 71, 504. Motokura, T., et al. 1991. Nature 350, 512. Solomon, M.J., et al. 1990. Cell 63, 1013. Draetta, G., and Beach, D. 1988. Cell 54, 17.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative control	trp E (Ab-1)
Positive control	MCF7, or A431 cells or breast carcinoma tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoblotting, Immunoprecipitation, and Neutralization Studies Lukas, J., et al. 1994. Oncogene 9, 707. Immunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Lukas, J., et al. 1994. Oncogene 9, 707. Paraffin and Frozen Sections Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Gillett, C., et al. 1994. Cancer Res. 54, 1812-1817.
Key Applications	Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Neutralization Studies Paraffin Sections
Application Notes	Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F) Frozen Sections (see application references) Immunoblotting (1 µg/ml) Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F) Immunoprecipitation (see application references) Neutralization Studies (see application references) Paraffin Sections (5 µg/ml, heat pre-treatment required)
Application Comments	Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant human cyclin D1
Immunogen	Human
Clone	DCS-6
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
CC12-100UGCN	04055977228465

Documentation

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) 分析证书

标题	批号
CC12	输入批号

参考

参考信息概述
Inaba, T., et al. 1992. Genomics 13, 565. Jiang, W., et al. 1992. Cancer Res. 2, 2980. Pines, J. 1992. Cell Growth Differ. 2, 305. Xiong, Y., et al. 1992. Genomics 13, 575. Xiong, Y., et al. 1992. Cell 71, 504. Motokura, T., et al. 1991. Nature 350, 512. Solomon, M.J., et al. 1990. Cell 63, 1013. Draetta, G., and Beach, D. 1988. Cell 54, 17.

引用

标题

Takeshi Shimamura, et al. (2005) Epidermal growth factor receptors harboring kinase domain mutations associate with the heat shock protein 90 chaperone and are destabilized following exposure to geldanamycins. Cancer Research 65, 6401-6408.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-August-2007 RFH
Synonyms	Anti-Bcl-1, Anti-PRAD-1
Application	Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F) Frozen Sections (see application references) Immunoblotting (1 µg/ml) Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F) Immunoprecipitation (see application references) Neutralization Studies (see application references) Paraffin Sections (5 µg/ml, heat pre-treatment required)
Description	Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
Background	Cyclins are a family of proteins implicated in the induction and control of mitosis. They are characterized by their accumulation and dramatic destruction in a cell cycle dependent manner. Cyclins are necessary for cdc/cdk kinase activity and are one of the subunits of the active kinase, in association with cdc2 or one of the cdk family members. Several different major cyclin families have been identified to date. These include the A, B, D and E family of cyclins. The D family of cyclins has been associated with a wide variety of proliferative diseases but their biochemical role is still unknown. The D cyclins appear in early G1, before the B cyclins. The human cyclin D1 gene has been mapped to the 11q13 locus and has been shown to be the PRAD1 gene product. PRAD1 was originally identified as being a clonally rearranged and overexpressed gene linked to the bcl-1 locus in a subset of benign parathyroid tumors. Amplification of cyclin D1 genes has also been demonstrated in human esophageal cancer.
Host	Mouse
Immunogen species	Human
Immunogen	recombinant human cyclin D1
Clone	DCS-6
Isotype	IgG_2a
Species	human, mouse, rat
Positive control	MCF7, or A431 cells or breast carcinoma tissue
Negative control	trp E (Ab-1)
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Inaba, T., et al. 1992. Genomics 13, 565. Jiang, W., et al. 1992. Cancer Res. 2, 2980. Pines, J. 1992. Cell Growth Differ. 2, 305. Xiong, Y., et al. 1992. Genomics 13, 575. Xiong, Y., et al. 1992. Cell 71, 504. Motokura, T., et al. 1991. Nature 350, 512. Solomon, M.J., et al. 1990. Cell 63, 1013. Draetta, G., and Beach, D. 1988. Cell 54, 17.
Citation	Takeshi Shimamura, et al. (2005) Epidermal growth factor receptors harboring kinase domain mutations associate with the heat shock protein 90 chaperone and are destabilized following exposure to geldanamycins. Cancer Research 65, 6401-6408.
Application references	Immunoblotting, Immunoprecipitation, and Neutralization Studies Lukas, J., et al. 1994. Oncogene 9, 707. Immunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Lukas, J., et al. 1994. Oncogene 9, 707. Paraffin and Frozen Sections Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Gillett, C., et al. 1994. Cancer Res. 54, 1812-1817.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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