数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABE873 Sigma-AldrichAnti-Chd2, clone 8H3 Antibody

This Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.

Anti-Chd2, clone 8H3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: Chd2 UniProt Number: E9PZM4

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IP, ICC, ChIP	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE873
Description	Anti-Chd2, clone 8H3 Antibody
Alternate Names	SNF2 chromatin remodelling enzyme Chromodomain Helicase DNA Binding Protein 2 Protein Chd2
Background Information	Chd2, also known as Chromodomain-helicase-DNA-binding protein 2 (CHD-2), or ATP-dependent helicase CHD2, and encoded by the gene CHD2, belongs to the CHD family of proteins. This family is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes are chromatin remodeling proteins and alter gene expression by modifying the chromatin structure of a particular gene. The alteration affects the access of the transcriptional apparatus to it, and thus the gene’s expression. In the mouse Chd2 is expressed in heart, forebrain, extremities, and facial and dorsal regions during specific times of embryonic development. Chd2 null mice are embryonic lethal, and in humans, various mutations in CHD-2 gene appear to cause a variety of disorders and developmental defects. Other mutations associated with the CHD2 gene locus appear to cause childhood-onset epileptic encephalopathy (EEOC).

References

Product Information
Format	Purified
Presentation	Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.
Key Applications	Western Blotting Immunoprecipitation Immunocytochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Chd2 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.). Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Biological Information
Immunogen	Recombinant protein corresponding to mouse Chd2.
Clone	8H3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rat
Isotype	IgG2aκ
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001074814
Gene Symbol	Chd2
Purification Method	Protein G Purified
UniProt Number	E9PZM4
Molecular Weight	~211 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. Two known isoforms may be observed at 50 kDa and 200 kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Chd2 in 10 µg of Jurkat cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE873	04053252955228

Documentation

Anti-Chd2, clone 8H3 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Chd2, clone 8H3 Antibody 分析证书

标题	批号
Anti-Chd2, clone 8H3 - Q2383914	Q2383914
Anti-Chd2, clone 8H3 - 3218874	3218874
Anti-Chd2, clone 8H3 - 3407335	3407335
Anti-Chd2, clone 8H3 - 3738081	3738081
Anti-Chd2, clone 8H3 - 3810505	3810505
Anti-Chd2, clone 8H3 - 3978231	3978231
Anti-Chd2, clone 8H3 - 4077670	4077670
Anti-Chd2, clone 8H3 - 4125701	4125701
Anti-Chd2, clone 8H3 - 4169254	4169254
Anti-Chd2, clone 8H3 -2659636	2659636

技术信息

标题
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。