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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABE1094 Sigma-Aldrich Anti-Cdx1 Antibody, clone 123a

Anti-Cdx1 Antibody, clone 123a is an antibody against Cdx1 for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).

Anti-Cdx1 Antibody, clone 123a MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: CDX1 UniProt Number: P47902

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, ChIP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE1094
Description	Anti-Cdx1 Antibody, clone 123a
Alternate Names	Homeobox protein CDX-1 Caudal-type homeobox protein 1
Background Information	Homeobox protein CDX-1 (UniProt P47902; also known as Caudal-type homeobox protein 1) is encoded by the CDX1 gene (Gene ID 1044) in human. CDX1 is a gut transcription factor crucial for colorectal differentiation by regulating the expression of structural proteins important for epithelial differentiation, including villin, cytokeratin 20, and FABP1. Exogenous expression of CDX1 in poorly differentiated cell lines that do not express endogenous CDX1 induces lumen formation in 3D cell cultures. CDX1 is upregulated in Barrett’s metaplasia of the esophagus and transgenic Cdx1 expression in mouse gastric epithelium causes intestinal transdifferentiation. CDX1 is transcriptionally silenced in many colorectal cancers due to promoter methylation.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Cdx1 Antibody, clone 123a is an antibody against Cdx1 for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).
Key Applications	Western Blotting Immunocytochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CdX1 in 10 µg of human stomach tissue lysate. Western Blotting Analysis: A representative lot detected the target band in CDX1-expressing colorectal cancer (CRC) cells (HT55, LS174T, RCM-1, SK-CO-1), but not in non-Cdx1-expressing CRC (DLD-1, HCT116, SW48, RKO) cells (Chan, C.W., et al. (2009). Proc. Natl. Acad. Sci .U. S. A. 106(6):1936-1941). Western Blotting Analysis: A representative lot detected the endogenously expressed CDX1 in HCT116 human colorectal cancer (CRC) cells. Antibody blocking with the immunogen peptide, but not with a C-terminal peptide, prevented the target band detection (Chan, C.W., et al. (2009). Proc. Natl. Acad. Sci .U. S. A. 106(6):1936-1941). Immunocytochemistry Analysis: A representative lot detected a downregulated CDX1 immunoreactivity among SW1222 and LS180 colorectal cancer (RC) cell colonies following prolyl-hydrolase inhibitor DMOG (Cat. No. 400091) treatment as a result of enhanced normoxia HIF-α transcription activity (Ashley, N., et al. (2013). Cancer Res. 73(18):5798-5809). Immunocytochemistry Analysis: A representative lot detected a high expression of the enterocyte differentiation marker CDX1 among colonies formed from colorectal cancer (RC) cells (SW1222, LS180, CCK-81) under normoxia condition, while a much lower Cdx1 immunostaining was seen among the colonies formed under hypoxia condition (Yeung, T.M., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(11):4382-4387). Immunocytochemistry Analysis: A representative lot immunostained LS174T and CDX1-transfected HCT116 colorectal cancer (CRC) cells. No staining was seen among mock-transfected HCT116 cells or CDX1 shRNA-transfected LS174T cells (Chan, C.W., et al. (2009). Proc. Natl. Acad. Sci. U. S. A. 106(6):1936-1941). Chromatin Immunoprecipitation ChIP) Analysis: A representative lot detected CDX1 occupancy at the KRT20 promoter site using chromatin preparation from HT55 human colorectal cancer (CRC) cells (Chan, C.W., et al. (2009). Proc. Natl. Acad. Sci. U. S. A. 106(6):1936-1941).

Biological Information
Immunogen	Linear peptide corresponding to a sequence from the N-terminal region of human Cdx1.
Epitope	N-terminal region.
Clone	C
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 123a detected the target band in CDX1-expressing colorectal cancer (CRC) cells, but not in non-CDX1-expressing CRC cells. Antibody blocking with the immunogen peptide, but not with a C-terminal peptide, prevented the detection of the target band (Chan, C.W., et al. (2009). Proc. Natl. Acad. Sci .U. S. A. 106(6):1936-1941). Immunogen sequence is not present in spliced isoform 2 reported by UniProt (P47902-2). Clone C is known to exhibit cross-reactivity toward an unidentified protein species in fibroblasts and smooth muscle cells. While such cross-reactivity is often not seen or extremely weak in epithelial cells, immunoreactivity by this clone must be interpreted carefully when handling samples of non-epithelial origins.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001795
Gene Symbol	CDX1
Purification Method	Protein G Purified
UniProt Number	P47902
Molecular Weight	~28-32 kDa observed. 28.14 kDa calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in human Caco-2 colorectal cancer cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cdx1 in 10 µg of human Caco-2 colorectal cancer cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE1094	04055977333282

Documentation

Anti-Cdx1 Antibody, clone 123a MSDS

职位
物料安全数据表 (MSDS)

Anti-Cdx1 Antibody, clone 123a 分析证书

标题	批号
Anti-Cdx1, clone 123a -Q2664690	Q2664690

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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