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Anti-Cathepsin D, rabbit polyclonal, recognizes the ~50 kDa latent and the ~30 kDa active forms in breast carcinoma cell line. It is validated for ELISA, Western blotting, and immunoelectrophoresis.
More>>Anti-Cathepsin D, rabbit polyclonal, recognizes the ~50 kDa latent and the ~30 kDa active forms in breast carcinoma cell line. It is validated for ELISA, Western blotting, and immunoelectrophoresis. Less<<
Anti-Cathepsin D Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D in breast carcinoma cell line.
Catalogue Number
219361
Brand Family
Calbiochem®
Application Data
Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
ELISA (≥1:1000) Immunoelectrophoresis (see comments)
Application Comments
Monospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
purified human liver cathepsin D
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
25-July-2007 RFH
Application
ELISA (≥1:1000) Immunoelectrophoresis (see comments)
Application Data
Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
Description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D.
Host
Rabbit
Immunogen species
Human
Immunogen
purified human liver cathepsin D
Isotype
IgG
Species
human
Positive control
Human liver tissue
Form
Liquid
Formulation
In PBS.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Monospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
