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Anti-Catalase, rabbit polyclonal, recognizes the ~60-65 kDa human and mouse catalase. It is validated for ELISA, Western blotting, and immunoelectrophoresis.
More>>Anti-Catalase, rabbit polyclonal, recognizes the ~60-65 kDa human and mouse catalase. It is validated for ELISA, Western blotting, and immunoelectrophoresis. Less<<
Anti-Catalase Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
Indirect ELISA (1:128) Immunoblotting (1:8000; see application references) Immunoelectrophoresis (see comments) Paraffin Sections (see application references)
Application Comments
The 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
purified, human erythrocytes catalase
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号
GTIN
219010-1MLCN
04055977202045
Documentation
Anti-Catalase Rabbit pAb 分析证书
标题
批号
219010
引用
标题
Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry282, 26245-26256.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
25-July-2007 RFH
Application
Indirect ELISA (1:128) Immunoblotting (1:8000; see application references) Immunoelectrophoresis (see comments) Paraffin Sections (see application references)
Application Data
Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
Description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. Recognizes the ~60-65 kDa (monomer MW) catalase protein under reducing conditions.
Host
Rabbit
Immunogen species
Human
Immunogen
purified, human erythrocytes catalase
Isotype
IgG
Species
human, mouse
Positive control
Red blood cells
Form
Liquid
Formulation
In PBS.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
The 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
Citation
Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry282, 26245-26256.
