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208910 Sigma-AldrichAnti-Calreticulin (405-417) Rabbit pAb

Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.

Anti-Calreticulin (405-417) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, Ca, Ht, H, Mk, M, Porcine, R	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
Catalogue Number	208910
Brand Family	Calbiochem®
Application Data	Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.

References
References	Janicki, S., et al. 1997. J. Cell Biol. 139, 485. Wada, I., et al. 1997. EMBO J. 16, 5420. Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741. Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603. Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.

Product Information
Form	Liquid
Formulation	In PBS, 50% glycerol, pH 7.2.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Immunohistochemistry Immunoprecipitation
Application Notes	Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent) Immunocytochemistry (see comments) Immunoprecipitation (see comments) Immunohistochemistry (see comments)
Application Comments	Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Biological Information
Immunogen	a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Bovine Canine Hamster Human Monkey Mouse Porcine Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
208910-50ULCN	04055977219524

Documentation

Anti-Calreticulin (405-417) Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

Anti-Calreticulin (405-417) Rabbit pAb 分析证书

标题	批号
208910	输入批号

参考

参考信息概述
Janicki, S., et al. 1997. J. Cell Biol. 139, 485. Wada, I., et al. 1997. EMBO J. 16, 5420. Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741. Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603. Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	20-April-2009 JSW
Application	Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent) Immunocytochemistry (see comments) Immunoprecipitation (see comments) Immunohistochemistry (see comments)
Application Data	Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
Description	Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
Background	Calreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. Calreticulin, which contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
Isotype	IgG
Species	bovine, canine, hamster, human, monkey, mouse, porcine, rat
Form	Liquid
Formulation	In PBS, 50% glycerol, pH 7.2.
Preservative	≤0.1% sodium azide
Comments	Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Janicki, S., et al. 1997. J. Cell Biol. 139, 485. Wada, I., et al. 1997. EMBO J. 16, 5420. Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741. Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603. Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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