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Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
More>>Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation. Less<<
Anti-Calbindin D-28K (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Frozen and Vibrotome Sections
Raven, M.A., et al. 2005. Vis. Neurosci.22, 569.
Key Applications
Frozen Sections
T Cell Co-stimulation
Application Notes
Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence) Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Application Comments
Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
purified bovine cerebellum calbindin D-28K protein
Immunogen
Bovine
Host
Rabbit
Isotype
IgG
Species Reactivity
Baboon
Human
Monkey
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-October-2007 RFH
Application
Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence) Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Description
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Background
Several EF-hand calcium-binding proteins have been identified within the nervous system, which includes calbindin, calretinin, and parvalbumin. These cytosolic calcium-binding proteins modulate and mediate the function of the intracellular second messenger calcium. These proteins are believed to function in the buffering, transport and regulation of calcium. Calbindin, calretinin, and parvalbumin are localized in distinct neuronal subpopulations, and antibodies against these proteins are useful markers for neuranatomical and developmental studies.
Host
Rabbit
Immunogen species
Bovine
Immunogen
purified bovine cerebellum calbindin D-28K protein
Isotype
IgG
Species
baboon, human, monkey, rat
Positive control
Rat striatum, hippocampus, or cortex
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
≤0.1% sodium azide
Comments
Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Conde, F., et al. 1994. J. Comp. Neurol.341, 95. Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15. Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303. Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.
