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Recognizes the CYP19A1 protein in human liver tissue.
Catalogue Number
ST1564
Brand Family
Calbiochem®
Synonyms
Anti-Cytochrome P450 19A1
Application Data
Detection of human CYP19A1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-CYP19A1 Rabbit pAb (Cat. No. ST1564) (1-5 µg/ml). Detection: chemiluminescence.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-Cytochrome P450 19A1
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human CYP19A1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-CYP19A1 Rabbit pAb (Cat. No. ST1564) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified rabbit polyclonal antibody. Recognizes the ~60 kDa CYP19A1 protein.
Background
CYP19A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation.
Host
Rabbit
Immunogen species
Human
Immunogen
Full-length, human CYP19A1 (aa 1-503)
Isotype
IgG₁
Species
human
Positive control
Human liver tissue
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
