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MABF981 Anti-CXCR4 Antibody, clone 12G5

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MABF981
100 μg  
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      概述

      Replacement Information

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, NEUTMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF981
      DescriptionAnti-CXCR4 Antibody, clone 12G5
      Alternate Names
      • C-X-C chemokine receptor type 4
      • CXC-R4
      • CXCR-4
      • FB22
      • HM89
      • LCR1
      • Leukocyte-derived seven transmembrane domain receptor
      • LESTR
      • Lipopolysaccharide-associated protein 3
      • LAP-3
      • LPS-associated protein 3
      • NPYRL
      • Stromal cell-derived factor 1 receptor
      • SDF-1 receptor
      • CXCR4
      • Fusin
      • CD184
      Background InformationC-X-C chemokine receptor type 4 (UniProt P61073; also known as CD184, CXC-R4, CXCR-4, FB22, Fusin, HM89, LAP-3, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, LPS-associated protein 3, Neuropeptide Y receptor Y3, NPYRL, SDF-1 receptor, Stromal cell-derived factor 1 receptor) is encoded by the CXCR4 (also known as D2S201E, HSY3RR, LAP3, NPY3R, NPYR, NPYY3R, WHIM, WHIMS) gene (Gene ID 7592) in human. Chemokines and their receptors direct migration of distinct leukocyte subsets to sites of inflammation and to their specific niches in lymphoid organs. Virtually all T cell chemoattractants selectively attract memory/activated T cells. CXCR4 is the seven-transmembrane receptor for SDF-1 that is essential for the development of non-hematopoietic tissues, including heart and B lymphocytes. CXCR4 and CCR5 are two chemokine receptors that also function as co-receptors for human immunodeficiency virus (HIV) entry into CD4+ cells. During the early stages of HIV infection, viral isolates tend to use CCR5 for viral entry, while later isolates tend to use CXCR4.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-CXCR4 Antibody, clone 12G5 is validated for use in Flow Cytometry, Immunocytochemistry and Neutralizing for the detection of CXCR4.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Neutralizing
      Application NotesFlow Cytometry Analysis: A representative lot detected surface expression of human CXCR4 in various cell types by flow cytometry (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930; McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696; Endres, M.J., et al. (1996). Cell. 87(4):745-756)
      Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed CHO cells transfected to stably express human CXCR4/fusin (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
      Neutralizing Analysis: A representative lot blocked SDF-1-induced lymphocyte chemotaxis using freshly isolated human PBMCs (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930).
      Neutralizing Analysis: A representative lot blocked the infection of CXCR4+/CD4+ rhabdomyosarcoma (RD) cells by seven HIV-1 (LAI, RF, Gun-1wt, Gun-1var) and HIV-2 (ROD A/B and CBL-23) strains as assessed by >95% reduced syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion. Clone 12G5 also blocked Gun-1wt, Gun-1var, and CBL-23, but not the other 4 HIV strains from infecting HeLa/CD4 and C8166 cultures (McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696).
      Neutralizing Analysis: A representative lot blocked HIV-2 /vcp strain from infecting U87 cells transfected to express human CXCR4/fusin as assessed a reduction of syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
      Note: Although clone 12G5 binds cell surface CXCR4, efforts to immunoprecipitate or immunoblot CXCR4 were reported to be unsuccessful (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
      Biological Information
      ImmunogenSimian immunodeficiency virus (SIVmac) variant CP-MAC-infected Sup-T1 human T lymphoblasts.
      EpitopeExtracellular domain
      Clone12G5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CXCR4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~39 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Jurkat cells.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected CXCR4 in Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABF981 04055977294170

      Documentation

      Anti-CXCR4 Antibody, clone 12G5 MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-CXCR4 Antibody, clone 12G5 分析证书

      标题批号
      Anti-CXCR4, clone 12G5 - 3324184 3324184
      Anti-CXCR4, clone 12G5 - 3389936 3389936
      Anti-CXCR4, clone 12G5 - 4159425 4159425
      Anti-CXCR4, clone 12G5 -Q2610505 Q2610505
      Anti-CXCR4, clone 12G5 Monoclonal Antibody 3143108

      参考

      参考概述公共医疗ID
      Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent.
      McKnight, A; Wilkinson, D; Simmons, G; Talbot, S; Picard, L; Ahuja, M; Marsh, M; Hoxie, JA; Clapham, PR
      Journal of virology  71  1692-6  1997

      显示摘要
      8995702 8995702
      The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.
      Bleul, CC; Wu, L; Hoxie, JA; Springer, TA; Mackay, CR
      Proceedings of the National Academy of Sciences of the United States of America  94  1925-30  1997

      显示摘要
      9050881 9050881
      CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.
      Endres, MJ; Clapham, PR; Marsh, M; Ahuja, M; Turner, JD; McKnight, A; Thomas, JF; Stoebenau-Haggarty, B; Choe, S; Vance, PJ; Wells, TN; Power, CA; Sutterwala, SS; Doms, RW; Landau, NR; Hoxie, JA
      Cell  87  745-56  1996

      显示摘要
      8929542 8929542

      小册子

      标题
      New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

      海报

      标题
      Post-Translational Modifications

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      种类

      Life Science Research > Antibodies and Assays > Primary Antibodies