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04-047 Sigma-AldrichAnti-CUGBP2 Antibody, clone 1H2

This Anti-CUGBP2 Antibody, clone 1H2 is validated for use in WB for the detection of CUGBP2.

Anti-CUGBP2 Antibody, clone 1H2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子

Research Category: Protein Trafficking Research Sub-Category: RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP) Gene Symbol: CUGBP2, CUG-BP2, BRUNOL3, CELF2, CELF-2, Etr-3, ETR-3, ETR3, NAPOR-2, NAPOR2, NAPOR, hNAPOR UniProt Number: O95319

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	04-047
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-CUGBP2 Antibody, clone 1H2

References

Product Information
Format	Purified
Presentation	100 μg of Protein A purified mouse monoclonal IgG1 in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Quality Level	MQ100

Applications
Application	This Anti-CUGBP2 Antibody, clone 1H2 is validated for use in WB for the detection of CUGBP2.
Key Applications	Western Blotting

Biological Information
Immunogen	A synthetic peptide corresponding to amino acids 209-415 of human CuGBP2.
Clone	clone 1H2
Host	Mouse
Specificity	CuGBP2
Isotype	IgG1
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001025076.2 NM_001025077.2 NM_001083591.1 NM_006561.3
Entrez Gene Summary	Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene Symbol	CUGBP2 CUG-BP2 BRUNOL3 CELF2 CELF-2 Etr-3 ETR-3 ETR3 NAPOR-2 NAPOR2 NAPOR hNAPOR
Purification Method	Protein A Purfied
UniProt Number	O95319
UniProt Summary	FUNCTION: SwissProt: O95319 # RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity). SIZE: 508 amino acids; 54285 Da SUBUNIT: Found in a complex with APOBEC1 and ACF. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and ACF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments. TISSUE SPECIFICITY: Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas. DEVELOPMENTAL STAGE: Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle. SIMILARITY: SwissProt: O95319 ## Belongs to the CELF/BRUNOL family. & Contains 3 RRM (RNA recognition motif) domains.
Molecular Weight	56 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C from date of shipment

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
04-047	04053252581625

Documentation

Anti-CUGBP2 Antibody, clone 1H2 MSDS

职位
物料安全数据表 (MSDS)

Anti-CUGBP2 Antibody, clone 1H2 分析证书

标题	批号
Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) - 2433335	2433335
Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1)	3066213
Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) - 2074888	2074888
Anti-CuGBP2, clone 1H2 - 3783547	3783547
Anti-CuGBP2, clone 1H2 - DAM1460230	DAM1460230
Anti-CuGBP2, clone 1H2 - DAM1817683	DAM1817683
Anti-CuGBP2, clone 1H2 - JBC1913123	JBC1913123
Anti-CuGBP2, clone 1H2 -2849462	2849462
Anti-CuGBP2, clone 1H2_2830872	2830872

参考

参考概述	公共医疗ID
ETR-3 and CELF4 protein domains required for RNA binding and splicing activity in vivo Singh, Gopal, et al Nucleic Acids Res, 32:1232-41 (2004) 2004	14973222
Dynamic antagonism between RNA-binding protein CUGBP2 and cyclooxygenase-2-mediated prostaglandin E2 in radiation damage Murmu, Nabendu, et al Proc Natl Acad Sci USA, 101:13873-8 (2004) 2004	15358864
CUGBP2 plays a critical role in apoptosis of breast cancer cells in response to genotoxic injury Mukhopadhyay, Debnath, et al Ann N Y Acad Sci, 1010:504-9 (2003) 2003	15033780
Expression and mutation analysis of BRUNOL3, a candidate gene for heart and thymus developmental defects associated with partial monosomy 10p Lichtner, P, et al J Mol Med, 80:431-42 (2002) 2002	12110949

小册子

标题
RNA-Binding Protein Immunoprecipitation

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描述

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种类

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