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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

217620 Sigma-AldrichAnti-CD64 Mouse mAb (10.1)

Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Anti-CD64 Mouse mAb (10.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~72 kDa CD64 protein (FcR1 receptor).
Catalogue Number	217620
Brand Family	Calbiochem®

References
References	Dougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Negative control	HPB-MLT
Positive control	4937 cells
Preservative	0.5 mg/ml gentamycin sulfate
Quality Level	MQ100

Applications
Key Applications	Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunoprecipitation Neutralization Studies
Application Notes	Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoblotting (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments)
Application Comments	The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵ cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	human rheumatoid synovial fluid cells/monocytes
Immunogen	Human
Clone	10.1
Host	Mouse
Purity	≥95% by SDS-PAGE
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
217620-100UGCN	04055977202915

Documentation

Anti-CD64 Mouse mAb (10.1) MSDS

职位
物料安全数据表 (MSDS)

Anti-CD64 Mouse mAb (10.1) 分析证书

标题	批号
217620	输入批号

参考

参考信息概述
Dougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-July-2007 RFH
Application	Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoblotting (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments)
Description	Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.
Host	Mouse
Immunogen species	Human
Immunogen	human rheumatoid synovial fluid cells/monocytes
Clone	10.1
Isotype	IgG₁
Species	human
Positive control	4937 cells
Negative control	HPB-MLT
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Purity	≥95% by SDS-PAGE
Preservative	0.5 mg/ml gentamycin sulfate
Comments	The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵ cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity	Standard Handling
References	Dougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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