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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

217595 Sigma-AldrichAnti-CD40L Mouse mAb (24-31)

Anti-CD40L, mouse monoclonal, clone 24-32, recognizes the ~36 kDa CD40L (gp39) on activated CD3+ human PBL. It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Anti-CD40L Mouse mAb (24-31) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
Data Sheet

同义词: Anti-gp39

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~36 kDa CD40L (gp39) protein on activated CD3+ human PBL.
Overview	Note: 1 T = 1 test.
Catalogue Number	217595
Brand Family	Calbiochem®
Synonyms	Anti-gp39

References

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative	0.5 mg/ml gentamycin sulfate
Quality Level	MQ100

Applications
Application References	Immunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.
Key Applications	Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunoprecipitation Neutralization Studies
Application Notes	Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments) Immunoblotting (see application references)
Application Comments	This antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x10⁵ ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	human sgp39 fusion protein
Immunogen	Human
Clone	24-31
Host	Mouse
Purity	≥95% by SDS-PAGE
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
217595-100UGCN	04055977202885

Documentation

Anti-CD40L Mouse mAb (24-31) MSDS

职位
物料安全数据表 (MSDS)

Anti-CD40L Mouse mAb (24-31) 分析证书

标题	批号
217595	输入批号

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-July-2007 RFH
Synonyms	Anti-gp39
Application	Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments) Immunoblotting (see application references)
Description	Protein A affinity purified mouse monoclonal antibody. Recognizes the ~39-40 kDa CD40L protein.
Background	Human CD40 ligand (CD40L) is a member of the tumor necrosis factor (TNF) family expressed on the surface of activated T-cells.
Host	Mouse
Immunogen species	Human
Immunogen	human sgp39 fusion protein
Clone	24-31
Isotype	IgG₁
Species	human
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Purity	≥95% by SDS-PAGE
Preservative	0.5 mg/ml gentamycin sulfate
Comments	This antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x10⁵ ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity	Standard Handling
Application references	Immunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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