Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Detect CD4 using this Anti-CD4 Antibody, intracellular, clone 024-10D6.B3 validated for use in ELISA, EMSA, FUNC & IC.
Key Applications
ELISA
Electrophoretic Mobility Shift Assay
Affects Function
Immunocytochemistry
Application Notes
Immunocytochemistry
ELISA
Stimulation/Inhibition of biological activity
Electrophoretic mobility shift analysis
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Synthetic peptide corresponding to amino acids 439-458 of the cytoplasmic tail of human CD4.
Epitope
intracellular
Clone
024-10D6.B3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes the cytoplasmic tail of CD4. Many functions have been assigned to this tail, including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transduction, among others. This antibody is useful as a control for studies involving targeting of intracellular protein domains of T cells.
FUNCTION: SwissProt: P01730 # Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. SIZE: 458 amino acids; 51111 Da SUBUNIT: Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. PTM: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. SIMILARITY: SwissProt: P01730 ## Contains 3 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. MISCELLANEOUS: Primary receptor for HIV-1.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at 2-8°C for up to 12 months from date of receipt.
Millipore’s CD4 Antibodies demonstrate specificity against CD4, a 55 kDa glycoprotein. See below for related products for CD4, based on the expertise of Upstate & Chemicon. 详细了解 >>
