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217570 Sigma-AldrichAnti-CD3 Mouse mAb (UCHT1)

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Anti-CD3 Mouse mAb (UCHT1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.
Catalogue Number	217570
Brand Family	Calbiochem®

References
References	Thibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814. Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative	0.5 mg/ml gentamycin sulfate
Quality Level	MQ100

Applications
Application References	T cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.
Key Applications	Flow Cytometry Frozen Sections Immunoprecipitation T Cell Co-stimulation
Application Notes	Flow Cytometry (5 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) T cell Co-stimulation (see application references)
Application Comments	This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 10⁵ cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	human thymocytes/Sezary T cells
Immunogen	Human
Clone	UCHT1
Host	Mouse
Purity	>95% immunoglobulin by SDS-PAGE
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
217570-100UGCN	04055977219418

Documentation

Anti-CD3 Mouse mAb (UCHT1) MSDS

职位
物料安全数据表 (MSDS)

Anti-CD3 Mouse mAb (UCHT1) 分析证书

标题	批号
217570	输入批号

参考

参考信息概述
Thibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814. Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.

引用

标题

Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-July-2007 RFH
Application	Flow Cytometry (5 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) T cell Co-stimulation (see application references)
Description	Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
Host	Mouse
Immunogen species	Human
Immunogen	human thymocytes/Sezary T cells
Clone	UCHT1
Isotype	IgG₁
Species	human
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Purity	>95% immunoglobulin by SDS-PAGE
Preservative	0.5 mg/ml gentamycin sulfate
Comments	This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 10⁵ cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity	Standard Handling
References	Thibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814. Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
Citation	Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
Application references	T cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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