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B-lymphocyte antigen CD20 (UniProt P11836; also known as B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1) is encoded by the MS4A1 (also known as CD20) gene (Gene ID 931) in human. CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines. The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface. It is believed that CD20 plays a direct role in mediating B cells proliferation and differentiation by regulating the transmembrane conductive Ca2+ flux.
References
Product Information
Format
AlexaFluor®647
Presentation
Purified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
This Anti-CD20 Antibody, clone FMC7, Alexa Fluor® 647 Conjugate is validated for use in Flow Cytometry for the detection of CD20.
Key Applications
Flow Cytometry
Application Notes
The unconjugated antibody (Cat. No. MAB1217) is shown to be suitable also for Flow cytometry and Western blotting applications.
Biological Information
Immunogen
The human B-lymphoblastoid cell line, HRIK.
Epitope
Extracellular domain
Clone
FMC7
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Originally reported to stain majority of prolymphocytic leukemia (B-PLL) and hairy-cell leukemia patients derived B cells (Catovsky, D., et al. (1981). Blood. 58(2):406-408.) and later reported to detect a 105 kDa protein species by immunoblotting (Zola, H., et al. (1987). Dis. Markers. 5(4):227-235.), clone FMC7 is now known to target a conformational epitope on the CD20 extracellular region. Clone FMC7 antigenicity is sensitive to levels of membrane cholesterol. Plamsa membrane cholesterol depletion by methyl-β-cyclodextrin treatment results in greatly reduced Ramos surface staining by clone FMC7 (Deans, J.P., and Polyak, M.J. (2008). Blood. 111(4):2492; author reply 2493-4; Polyak, M.J., et al. (2003). Leukemia. 17(7):1384-1389; Serke, S., et al. (2001). Cytometry. 46(2):98-104).
Flow Cytometry Analysis: 1.0 µg of this antibody detected CD20 in human PBMCs.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
