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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABN889 Sigma-AldrichAnti-C9ORF72/C9RANT (poly-GA) Antibody, clone 5E9

Detect C9ORF72/C9RANT using this Anti-C9ORF72/C9RANT, clone 5E9 Antibody validated for use in Western Blotting and Immunohistochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: C9orf72 UniProt Number: Q96LT7

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概述

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	IHC, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN889
Description	Anti-C9ORF72/C9RANT (poly-GA) Antibody, clone 5E9
Alternate Names	C9ORF72/C9RANT (poly-GA) Protein C9orf72
Background Information	Expansion of a GGGGCC hexanucleotide repeat sequence in the non-coding region of human chromosome 9 open reading frame 72 or C9orf72 (also known as ALSFTD, FTDALS; Gene ID 203228) is the most common genetic abnormality in familial and sporadic frontotemporal dementia (FTD) and motor neuron disease (MND), with amyotrophic lateral sclerosis (ALS) as the most frequent form. The number of hexanucleotide repeats in the normal population ranges from 2 to 24, whereas up to several thousand repeats (700 - 4,400 repeats) are found in the pathologically expanded allele. Unconventional repeat-associated non-ATG-initiated translation (RANT) of the GGGGCC repeats in the three alternate reading frames generates three polypeptides, each composed of repeating units of two amino acids (dipeptide repeats, DPRs), glycine-alanine (GA), glycine-proline (GP), and glycine-arginine (GR), respectively. These DPR proteins form inclusions particularly in the cerebellar cortex, hippocampus and cerebral neocortex. These inclusions are also immunoreactive for markers of the ubiquitin proteasome system (UPS), including ubiquitin, ubiquilins, and p62, but is distinct from inclusions containing TDP-43 that is also found in FTLD-TDP (frontotemporal lobar degeneration with TDP-43 pathology) and ALS cases. Antibodies against poly-GA, poly-GP, and poly-GR sequence are useful for characterizing the neuroanatomical distribution and clinico-pathological association of DPR pathology with C9ORF72 mutations.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect C9ORF72/C9RANT using this Anti-C9ORF72/C9RANT, clone 5E9 Antibody validated for use in Western Blotting and Immunohistochemistry.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Western Blotting Analysis: A representative lot detected recombinant GST fusion with 15 GA repeats (Mackenzie, I.R., et al. (2013). Acta Neuropahol. 126(6):859-879). Immunohistochemistry Analysis: A representative lot detected C9ORF72/C9RANT with 15 GA repeats in cerebellum from a C9ORF72 mutation case (Mackenzie, I.R., et al. (2013). Acta Neuropahol. 126(6):859-879).

Biological Information
Immunogen	Polyethylene glycol-conjugated linear peptide containing poly-GA sequence.
Epitope	Poly-GA
Clone	5E9
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	This antibody recognizes C9ORF72/C9RANT (poly-GA) and other proteins containing poly-GA sequence.
Isotype	IgG1κ
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to react with all species
Antibody Type	Monoclonal Antibody
Gene Symbol	C9orf72
Purification Method	Protein G Purified
UniProt Number	Q96LT7
Molecular Weight	Variable. Additional uncharacterized bands maybe observed in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western blotting using GST fusion recombinant protein with 15 GA repeats. Western Blotting Analysis: 0.05 µg/mL of this antibody detected 10 ug of GST fusion recombinant protein with 15 GA repeats
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	50 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN889	04055977171426

Documentation

Anti-C9ORF72/C9RANT (poly-GA) Antibody, clone 5E9 MSDS

职位
物料安全数据表 (MSDS)

Anti-C9ORF72/C9RANT (poly-GA) Antibody, clone 5E9 分析证书

标题	批号
Anti-C9ORF72/C9RANT (poly-GA), -2653164	2653164
Anti-C9ORF72/C9RANT (poly-GA), -2794074	2794074
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3150354	3150354
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3226534	3226534
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3328868	3328868
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3470729	3470729
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3557714	3557714
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3694727	3694727
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3738412	3738412
Anti-C9ORF72/C9RANT (poly-GA), clone 5E9 - 3808623	3808623

技术信息

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White Paper: Further considerations of antibody validation and usage.

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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