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Anti-BCL9, mouse monoclonal, clone 2D4, recognizes the BCL9 protein in HeLa cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
More>>Anti-BCL9, mouse monoclonal, clone 2D4, recognizes the BCL9 protein in HeLa cells. It is validated for ELISA, Western blotting, and immunocytochemistry. Less<<
Anti-BCL9 Mouse mAb (2D4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 1036-1136 human BCL9, expressed as a GST fusion protein
Clone
2D4
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 RFH
Synonyms
Anti-β-cell CLL/Lymphoma 9
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Description
Purified mouse monoclonal antibody. Recognizes the BCL9 protein.
Background
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 1036-1136 human BCL9, expressed as a GST fusion protein
Clone
2D4
Isotype
IgG₁
Species
human
Positive control
HeLa cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
