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MABE144 Sigma-AldrichAnti-Ago3 Antibody, clone 4B1-F6

Anti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.

Anti-Ago3 Antibody, clone 4B1-F6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
小册子

Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: EIF2C3, AGO3 UniProt Number: Q9H9G7

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ICC, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE144
Description	Anti-Ago3 Antibody, clone 4B1-F6
Alternate Names	eIF-2C 3 eIF2C 3 FLJ12765 MGC86946
Background Information	Eukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.

References

Product Information
Format	Purified
Control	HeLa cells
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.
Key Applications	Immunocytochemistry Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Azuma-Mukai, A., et al. (2008). Proc Natl Acad Sci. USA. 105(23):7964-7969.)

Biological Information
Immunogen	Recombinant protein corresponding to human Ago3.
Clone	4B1-F6
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody has been shown to not cross react with Ago1, Ago2, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
Isotype	IgG2aκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_079128
Entrez Gene Summary	This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene.
Gene Symbol	EIF2C3 AGO3
Purification Method	Protein G Purified
UniProt Number	Q9H9G7
UniProt Summary	FUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. SUBUNIT STRUCTURE: Interacts with EIF4B, IMP8, PRMT5 and TNRC6B. SUBCELLULAR LOCATION: Cytoplasm > P-body.
Molecular Weight	96 kDa calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Ago3 in HeLa cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE144	04053252754395

Documentation

Anti-Ago3 Antibody, clone 4B1-F6 MSDS

职位
物料安全数据表 (MSDS)

Anti-Ago3 Antibody, clone 4B1-F6 分析证书

标题	批号
Anti-Ago3, clone 4B1-F6 - 2391171	2391171
Anti-Ago3, clone 4B1-F6 - 2006946	2006946
Anti-Ago3, clone 4B1-F6 - 3310131	3310131
Anti-Ago3, clone 4B1-F6 - 3433738	3433738
Anti-Ago3, clone 4B1-F6 - 3808724	3808724
Anti-Ago3, clone 4B1-F6 - NRG1911092	NRG1911092
Anti-Ago3, clone 4B1-F6 -2524850	2524850
Anti-Ago3, clone 4B1-F6 -2775321	2775321
Anti-Ago3, clone 4B1-F6 -2786916	2786916
Anti-Ago3, clone 4B1-F6 Monoclonal Antibody	2929151

小册子

标题
New Products: Volume 3, 2012

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描述

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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