数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABE387 Sigma-AldrichAnti-ATF4 Antibody

Anti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP.

Anti-ATF4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: ATF4, CREB2, TXREB UniProt Number: P18848

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ChIP	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	ABE387
Description	Anti-ATF4 Antibody
Alternate Names	Cyclic AMP-dependent transcription factor ATF-4 cAMP-dependent transcription factor ATF-4 Activating transcription factor 4 Cyclic AMP-responsive element-binding protein 2 CREB-2 cAMP-responsive element-binding protein 2 DNA-binding protein TAXREB67 Tax-responsive enhancer element-binding protein 67 TaxREB67
Background Information	Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.

References

Product Information
Format	Serum
Control	HisOH treated HepG2 cell lysate
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP.
Key Applications	Western Blotting Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 cell lysates (Su, N. and Kilberg, M. S., et al. (2008). J Biol Chem. 283(50):351016-35117.; Fu, L., et al. (2011). J Biol Chem. 286(42):36724-36738.). Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 and MEF cell lysates (Shan, J., et al. (2012). J Biol Chem. 287(43):36393-36403.).

Biological Information
Immunogen	Recombinant protein corresponding to human ATF4.
Host	Rabbit
Isotype	IgG
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001666.2
Gene Symbol	ATF4 CREB2 TXREB
Purification Method	Unpurified
UniProt Number	P18848
Molecular Weight	~48 kDa observed. The calculated molecular weight is 39 kDa; however, this protein may be observed at ~48 kDa in human tissue lysates and ~45 kDa in mouse cell lysates due to post-translational modification. Uncharacterized bands may be observed at ~57 kDa, ~59 kDa, ~75 kDa, and ~100 kDa in some cell lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in untreated and HisOH treated HepG2 cell lysate. Western Blot Analysis: A 1:2,000 dilution from a representative lot detected ATF4 in 10 µg of HisOH treated HepG2 cell lysate (HisOH was used to induce cellular stress mediated eIF2a phosphorylation, allowing for subsequent detection of ATF4. For enhanced performance, end-users may try higher dilutions of this antibody. For better results, 1% SDS was used during the secondary antibody incubation step.)
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in untreated and HisOH treated HepG2 cell lysate.

Western Blot Analysis: A 1:2,000 dilution from a representative lot detected ATF4 in 10 µg of HisOH treated HepG2 cell lysate (HisOH was used to induce cellular stress mediated eIF2a phosphorylation, allowing for subsequent detection of ATF4. For enhanced performance, end-users may try higher dilutions of this antibody. For better results, 1% SDS was used during the secondary antibody incubation step.)

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABE387	04053252850769

Documentation

Anti-ATF4 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-ATF4 Antibody 分析证书

标题	批号
Anti-ATF4	3078493
Anti-ATF4 - 3398042	3398042
Anti-ATF4 - 3495620	3495620
Anti-ATF4 - 3707450	3707450
Anti-ATF4 - 4070112	4070112
Anti-ATF4 - Q2116528	Q2116528
Anti-ATF4 -2501504	2501504
Anti-ATF4 -2736396	2736396
Anti-ATF4 Polyclonal Antibody	3037998
Anti-ATF4 Polyclonal Antibody	3120174

技术信息

标题
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。