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Anti-ARSA, mouse polyclonal, recognizes the ~55 kDa ARSA protein in human lymph node tissue. It is validated for Western blotting and paraffin sections.
More>>Anti-ARSA, mouse polyclonal, recognizes the ~55 kDa ARSA protein in human lymph node tissue. It is validated for Western blotting and paraffin sections. Less<<
Anti-ARSA Mouse pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human ARSA by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with human ARSA (lane 1) non-transfected 293T cells (lane 2). Primary antibody: Anti-ARSA Mouse pAb (Cat. No. AP1138) (1-5 µg/ml). Detection: chemiluminescence. Detection of human ARSA by staining paraffin sections. Sample: Formalin/PFA-fixed, paraffin-embedded human lymph node tissue. Primary antibody: Anti-ARSA Mouse pAb (Cat. No. AP1138) (3 µg/ml). Detection: colrimetric (DAB).
Description
Purified mouse polyclonal antibody. Recognizes the ~55 kDa ARSA protein.
Background
ARSA hydrolyzes cerebroside sulfate. Defects in ARSA are known to cause metachromatic leukodystrophy (MLD). MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Host
Mouse
Immunogen species
Human
Immunogen
Full length human ARSA (aa 1-507)
Isotype
IgG
Species
human
Positive control
Human lymph node tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
