数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

05-1427 Sigma-AldrichAnti-ARF1 Antibody, clone 3F1

Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).

Anti-ARF1 Antibody, clone 3F1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ARF1 UniProt Number: P84077

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information
Replacement Information	05-1427 is a recommended replacement for MAB3779

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ELISA, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-1427
Replaces	MAB3779
Description	Anti-ARF1 Antibody, clone 3F1
Alternate Names	ADP-ribosylation factor 1
Background Information	ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.

References

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide
Quality Level	MQ100

Applications
Application	Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
Key Applications	Western Blotting ELISA Immunohistochemistry (Paraffin)
Application Notes	ELISA: A previous lot of this antibody was used in ELISA. Optimal working dilutions must be determined by end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer

Biological Information
Immunogen	Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
Epitope	C-terminus
Clone	3F1
Host	Mouse
Specificity	Recognizes human ARF1
Isotype	IgG2a
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001024226.1
Entrez Gene Summary	ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene Symbol	ARF1
Purification Method	Purified
UniProt Number	P84077
UniProt Summary	FUNCTION: SwissProt: P84077 # GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. SIZE: 181 amino acids; 20697 Da SUBUNIT: Interacts with PRKCABP, GGA1, DDEF2 and PIP5K1A (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Binds DDEF2. Interacts with ARHGAP21. SIMILARITY: SwissProt: P84077 ## Belongs to the small GTPase superfamily. Arf family
Molecular Weight	~21 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on HeLa lysates. Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
05-1427	04053252277665

Documentation

Anti-ARF1 Antibody, clone 3F1 MSDS

职位
物料安全数据表 (MSDS)

Anti-ARF1 Antibody, clone 3F1 分析证书

标题	批号
Anti-ARF1, clone 3F1 - NG1590610	NG1590610
Anti-ARF1, clone 3F1 - NG1590617	NG1590617
Anti-ARF1, clone 3F1, C-terminus - 2116005	2116005
Anti-ARF1, clone 3F1, C-terminus - 2128248	2128248
Anti-ARF1, clone 3F1, C-terminus - 2153064	2153064
Anti-ARF1, clone 3F1, C-terminus - 2272153	2272153
Anti-ARF1, clone 3F1, C-terminus - 3427188	3427188
Anti-ARF1, clone 3F1, C-terminus - NG1726950	NG1726950
Anti-ARF1, clone 3F1, C-terminus - NG1943280	NG1943280
Anti-ARF1, clone 3F1, C-terminus -2557580	2557580

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。