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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABN59 Sigma-AldrichAnti-ACE2 Antibody, clone 4G5.1

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.

Anti-ACE2 Antibody, clone 4G5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: ACE2 UniProt Number: Q9BYF1

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN59
Replaces	MAB5676
Description	Anti-ACE2 Antibody, clone 4G5.1
Alternate Names	Angiotensin-converting enzyme 2 ACE-related carboxypeptidase Angiotensin-converting enzyme homolog ACEH Metalloprotease MPROT15
Background Information	ACE2 (Angiotensin-converting enzyme 2) is a transmembrane protein belonging to the peptidase M2 family. It is expressed in vascular endothelial cells of the heart, kidney, testis, and gastrointestinal system. ACE2 produces Angiotensin (1-9) by hydrolysis of Angiotensin 1. Angiotensin (1-9) is a precursor for Angiotensin (1-7). ACE2 may also directly produce Angiotensin (1-7) by conversion of Angiotensin II, which is involved in vasoconstriction. Angiotensin (1-7) plays an important role in the RAS signaling pathway and is involved in vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as linosipril and captopril. ACE2 is upregulated in heart failure, possibly in response to overactivity of Angiotensin II; ACE2 is studied for its potential therapeutic value for hypertension and heart failure.

References

Product Information
Format	Purified
Control	HEK293 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.
Key Applications	Western Blotting
Application Notes	ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human ACE2.
Clone	4G5.1
Host	Mouse
Specificity	This antibody recognizes ACE2.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_068576
Entrez Gene Summary	The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Gene Symbol	ACE2
Purification Method	Protein G Purified
UniProt Number	Q9BYF1
UniProt Summary	FUNCTION: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. CATALYTIC ACTIVITY: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine. COFACTOR: Binds 1 zinc ion per subunit. Binds 1 chloride ion per subunit. ENZYME REGULATION: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. SUBUNIT STRUCTURE: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein. SUBCELLULAR LOCATION: Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. INDUCTION: Up-regulated in failing heart. POST-TRANSLATIONAL MODIFICATION: N-glycosylation on Asn-90 may limit SARS infectivity. SEQUENCE SIMILARITIES: Belongs to the peptidase M2 family.
Molecular Weight	~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN59	04053252643415

Documentation

Anti-ACE2 Antibody, clone 4G5.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-ACE2 Antibody, clone 4G5.1 分析证书

标题	批号
Anti-ACE2, clone 4G5.1	2487732
Anti-ACE2, clone 4G5.1 - 2031360	2031360
Anti-ACE2, clone 4G5.1 - 3257585	3257585
Anti-ACE2, clone 4G5.1 - 3439690	3439690
Anti-ACE2, clone 4G5.1 - 3451422	3451422
Anti-ACE2, clone 4G5.1 - 3465732	3465732
Anti-ACE2, clone 4G5.1 - 3476046	3476046
Anti-ACE2, clone 4G5.1 - 3499346	3499346
Anti-ACE2, clone 4G5.1 - 3508402	3508402
Anti-ACE2, clone 4G5.1 - 3537157	3537157

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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