数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

AM63 Sigma-AldrichAnti-A20 Mouse mAb (59A426)

Anti-A20 mouse monoclonal antibody, clone 59A426, recognizes ~70 kDa A20 protein in Daudi cells. It is validated for use in immunoblotting and immunoprecipitation.

Anti-A20 Mouse mAb (59A426) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~70 kDa A20 protein in Daudi cells.
Catalogue Number	AM63
Brand Family	Calbiochem®
Application Data	Detection of human A20 by immunoblotting. Sample: Whole cell lysate (10 µg) from Daudi cells. Primary antibody: Anti-A20 Mouse mAb (59A426) (Cat. No. AM63) (3 µg/ml). Detection: chemiluminescence.

References
References	Heyninck, K., et al. J. Cell Biol 145, 1471. May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63. Tewari, M., et al. 1995. J. Immunol. 154, 1699. Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.

Product Information
Form	Liquid
Formulation	In PBS, 0.2% gelatin.
Positive control	Daudi cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunoprecipitation
Application Notes	Immunoblotting (1:500-1:2000) Immunoprecipitation (2 µg/mg)
Application Comments	Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	full-length recombinant human A20 protein
Immunogen	Human
Clone	59A426
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
AM63-100UGCN	04055977228021

Documentation

Anti-A20 Mouse mAb (59A426) MSDS

职位
物料安全数据表 (MSDS)

Anti-A20 Mouse mAb (59A426) 分析证书

标题	批号
AM63	输入批号

参考

参考信息概述
Heyninck, K., et al. J. Cell Biol 145, 1471. May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63. Tewari, M., et al. 1995. J. Immunol. 154, 1699. Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.

小册子

标题
Antibody Sourcebookﾙ Sixth Edition
Caspases and other Apoptosis Related Tools Brochure

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	17-August-2007 RFH
Application	Immunoblotting (1:500-1:2000) Immunoprecipitation (2 µg/mg)
Application Data	Detection of human A20 by immunoblotting. Sample: Whole cell lysate (10 µg) from Daudi cells. Primary antibody: Anti-A20 Mouse mAb (59A426) (Cat. No. AM63) (3 µg/ml). Detection: chemiluminescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~70 kDa A20 protein.
Background	NF-κB (nuclear factor κB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-κB gene expression by interferring with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IκB kinase pathway. A20, an 70 kDa protein, is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues.
Host	Mouse
Immunogen species	Human
Immunogen	full-length recombinant human A20 protein
Clone	59A426
Isotype	IgG₁
Species	human
Positive control	Daudi cells
Form	Liquid
Formulation	In PBS, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot freeze (-20°C).
Toxicity	Standard Handling
References	Heyninck, K., et al. J. Cell Biol 145, 1471. May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63. Tewari, M., et al. 1995. J. Immunol. 154, 1699. Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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