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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

NA02A Sigma-AldrichAnti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
Catalogue Number	NA02A
Brand Family	Calbiochem®

References
References	Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.

References

Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.

Product Information
Form	Liquid suspension
Formulation	In PBS.
Negative control	Transfer RNA (tRNA)
Positive control	Heterogenous nuclear RNA (hnRNA)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Affinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.
Key Applications	Affinity Purification Immunoblotting (Western Blotting)
Application Notes	Affinity Purification (see application references) Immunoprecipitation (15 µl beads/sample) Immunoprecipitation (1-2 µg/ml; see application references) Immunocytochemistry (use Cat. No. NA02) Paraffin Sections (use Cat. No. NA02)
Application Comments	Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.

Biological Information
Immunogen	2,2,7-trimethylguanosine
Clone	K121
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
NA02A-0.5MLCN	04055977227611

Documentation

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate MSDS

职位
物料安全数据表 (MSDS)

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate 分析证书

标题	批号
NA02A	输入批号

参考

参考信息概述
Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.

参考信息概述

小册子

标题
Antibody Sourcebookﾙ Sixth Edition

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	04-September-2007 RFH
Application	Affinity Purification (see application references) Immunoprecipitation (15 µl beads/sample) Immunoprecipitation (1-2 µg/ml; see application references) Immunocytochemistry (use Cat. No. NA02) Paraffin Sections (use Cat. No. NA02)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5' cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Background	The U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
Host	Mouse
Immunogen	2,2,7-trimethylguanosine
Clone	K121
Isotype	IgG₁
Species	human, mouse, rat
Positive control	Heterogenous nuclear RNA (hnRNA)
Negative control	Transfer RNA (tRNA)
Form	Liquid suspension
Formulation	In PBS.
Preservative	≤0.1% sodium azide
Comments	Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.
Application references	Affinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.

Related Products

产品目录编号

描述

NA02

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121)

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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