It appears that your browser has JavaScript disabled.

This Website requires your browser to be JavaScript enabled.

Please enable JavaScript and reload this page.

NA02 Sigma-AldrichAnti-2,2,7-Trimethylguanosine Mouse mAb (K121)

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) is validated for use in Affinity Purification, ICC, IP, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
小册子
Data Sheet

Recommended Products

概述

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
NA02-100UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	100 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Catalogue Number	NA02
Brand Family	Calbiochem®
Application Data	Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.

References
References	Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative control	Transfer RNA (tRNA)
Positive control	Heterogenous nuclear RNA (hnRNA)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Affinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.
Key Applications	Immunocytochemistry Immunoprecipitation Paraffin Sections
Application Notes	Affinity Purification (use Cat. No. NA02A, see application references) Immunocytochemistry (0.5-5 µg/ml) Immunoprecipitation (1-2 µg, see application references) Paraffin Sections (see application references)
Application Comments	Also recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	2,2,7-trimethylguanosine
Clone	K121
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Global Trade Item Number
产品目录编号	GTIN
NA02-100UGCN	07790788053840

最近查看的内容