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Recombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
Catalogue Number
176908
Brand Family
Calbiochem®
Synonyms
PA 63
References
References
Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7045. Nassi, S., et al. 2002. Biochemistry41, 1445. Singh, Y., et al. 1999. Infect. Immun.67, 1853. Leppla, S.H. 1988. Methods Enzymol.165, 103.
Product Information
ATP Competitive
N
Form
Lyophilized
Formulation
Lyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
Following reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7045. Nassi, S., et al. 2002. Biochemistry41, 1445. Singh, Y., et al. 1999. Infect. Immun.67, 1853. Leppla, S.H. 1988. Methods Enzymol.165, 103.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
01-September-2009 RFH
Synonyms
PA 63
Description
Recombinant, Bacillus anthracis anthrax protective antigen(nicked) expressed in a special strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
Form
Lyophilized
Formulation
Lyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
Purity
Single major band at 63 kD by SDS-PAGE
Solubility
Reconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
Toxicity
Standard Handling
References
Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7045. Nassi, S., et al. 2002. Biochemistry41, 1445. Singh, Y., et al. 1999. Infect. Immun.67, 1853. Leppla, S.H. 1988. Methods Enzymol.165, 103.
