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General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.2. Mesh: <50 (minimum 80%).
Catalogue Number
194932
Brand Family
Calbiochem®
References
References
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997. Moore, G.L. 1969. Anal. Biochem. 32, 122.
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997. Moore, G.L. 1969. Anal. Biochem. 32, 122.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-November-2009 RFH
Description
General proteolytic substrate. Provides a rapid and simple spectrophotometric method to detect the presence of proteases in aqueous solutions. As the insoluble substrate is cleaved by proteases, it releases a red dye into the surrounding buffer. Incubation of 50 mg AZOCOLL® Substrate with 2 units of PRONASE® Protease for 15 min yields an absorbance of greater than 1.0 at 520 nm.
Form
Dark red solid
CAS number
53092-90-3
Solubility
H₂O or Aqueous Buffers
Storage
Protect from light
+15°C to +30°C
Do Not Freeze
Ok to freeze
Toxicity
Standard Handling
References
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997. Moore, G.L. 1969. Anal. Biochem. 32, 122.
