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194933 Sigma-AldrichAZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem

General proteolytic substrate.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

Recommended Products

概述

Replacement Information

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
194933-1GMCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	1 gm	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹
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Description
Overview	General proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.3. Mesh: >100.
Catalogue Number	194933
Brand Family	Calbiochem®

References
References	Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.

Product Information
CAS number	53092-90-3
ATP Competitive	N
Form	Dark red solid
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Primary Target	General proteolytic substrate

Physicochemical Information
Cell permeable	N

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+15°C to +30°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
194933-1GMCN	04055977206401
194933-5GMCN	07790788048266

Documentation

AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem 分析证书

标题	批号
194933	输入批号

参考

参考信息概述
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	31-October-2016 JSW
Description	General proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 5 mg/ml buffer, 10 min at 37°C, pH 7.0): <0.3. Mesh: >100.
Form	Dark red solid
Recommended reaction conditions	Assay Protocol for Protease Activity 1. Dissolve 50 mg AZOCOLL^® in 5 ml of buffer solution (e.g., PBS, pH 7.0) containing the proteolytic enzyme. 2. Incubate in stoppered tubes for 15 min at 37°C, agitating gently at 5 min intervals. 3. Cool the tubes in ice water and filter the contents through Whatman No. 1 filter paper. 4. Measure the absorbance at 520 nm, subtracting the blank value. 5. Plot absorbance versus protease activity. A linear relationship exists between protease concentration and the rate of dye liberation. Note: The rate of color yield from AZOCOLL^® will vary with the individual proteases tested. This protocol is meant to serve as a guideline and may need to be modified for specific applications.
CAS number	53092-90-3
Solubility	PBS (10 mg/ml)
Storage	Protect from light +15°C to +30°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Standard Handling
References	Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.

种类

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Protease Substrates

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