Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
ABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.
Form
Clear to very pale yellow solution
Formulation
Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
Recommended reaction conditions
Suggested procedure for use of ABTS in HRP-based ELISAs:
1. Complete all required incubations with antibodies and HRP-labeled probes.
2. Wash plate wells at least 4 times with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween®-20 detergent.
3. After the final wash, shake and blot all residual buffer from the wells.
4. Add 100 µl of ABTS Solution to each well and incubate at room temperature for up to 30 min. (If a kinetic study is desired, readings at 405 nm may be taken at intermediate intervals.)
Note: Optimal incubation times may vary depending on the amount of HRP present. If color develops too quickly, zero order kinetics will not prevail. Dilution of the probe, antibody, or HRP-labeled reagent may be required.
5. Add 100 µl of stop solution, mix well, and read absorbance at 405 nm. [A 0.625 M oxalic acid solution is recommended as a stopping solution, as the colored product is most stable with this reagent (at least 1 h). Other acids may be employed (0.625 M oxalic acid > 0.5 M H2SO4 > 0.5 M HCl). 1% SDS may also be used as a stopping solution, provided that it is pure and clear (impure or turbid SDS solutions may interfere with absorbance readings).]
Note: Variations in time, reagent volumes, and temperature may require further standardization by the user.
CAS number
28752-68-3
Chemical formula
C₁₈H₁₈N₄O₆S₄
Preservative
≤0.1% sodium azide
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
ABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
Toxicity
Irritant
References
Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.
