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437996 Sigma-Aldrich5-Lipoxygenase, Human, Recombinant, S. fruigiperda

5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: 5-LO

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概述

Replacement Information

价格及供货情况

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Description
Overview	Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A₄. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA₂.
Catalogue Number	437996
Brand Family	Calbiochem®
Synonyms	5-LO

References
References	Brock, T.G., et al. 1997. J. Biol. Chem. 272, 8276. Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250. Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535. Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.

Product Information
Unit of Definition	One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
EC number	1.13.11.34
Form	Liquid
Formulation	In 100 mM Tris containing 5 mM EGTA, pH 8.0.
Quality Level	MQ100

Applications

Biological Information
Specific Activity	~20 units/mg

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
437996-500UCN	04055977186901

Documentation

5-Lipoxygenase, Human, Recombinant, S. fruigiperda MSDS

职位
物料安全数据表 (MSDS)

5-Lipoxygenase, Human, Recombinant, S. fruigiperda 分析证书

标题	批号
437996	输入批号

参考

参考信息概述
Brock, T.G., et al. 1997. J. Biol. Chem. 272, 8276. Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250. Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535. Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	06-August-2008 RFH
Synonyms	5-LO
Description	Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. It is localized in the cytosol of resting neutrophils, and is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA₂.
Form	Liquid
Formulation	In 100 mM Tris containing 5 mM EGTA, pH 8.0.
EC number	1.13.11.34
Specific activity	~20 units/mg
Unit definition	One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).
Toxicity	Standard Handling
References	Brock, T.G., et al. 1997. J. Biol. Chem. 272, 8276. Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250. Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535. Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.

种类

Life Science Research > Proteins and Enzymes > Other Enzymes

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