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Recombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Catalogue Number
407321
Brand Family
Calbiochem®
References
References
Alvarado, J., et al. 2005. Immunol. Lett.99, 109. Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499. Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
Reported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
Purity
≥95% by RP-HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Protect from Moisture
Protect from moisture
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Alvarado, J., et al. 2005. Immunol. Lett.99, 109. Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499. Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-July-2010 JSW
Description
Recombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Form
Lyophilized
Formulation
Lyophilized from 0.5X PBS, pH 7.2.
Purity
≥95% by RP-HPLC
Biological activity
Reported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
Solubility
Reconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
Storage
Protect from moisture
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Alvarado, J., et al. 2005. Immunol. Lett.99, 109. Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499. Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
